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. 2015 Jul 9;10(7):e0131711. doi: 10.1371/journal.pone.0131711

Fig 1. Nacrein promoter is transcriptionally active.

Fig 1

Different amounts of pGL3-Nacrein plasmid (0, 0.4 μg, 0.8 μg, 1.6 μg) was transfected into Hela cells and promoter-dependent Luciferase activity was measured. We used Renilla as an internal reference. Empty pGL-3 Basic plasmid was used to keep the total amount of plasmid the same in each group. Each reaction was completed in triplicate. Luciferase activity increased as the dose of pGL3-Nacrein luciferase vector increased, confirming that the constructed pGL3-Nacrein promoter plasmid is transcriptionally active. Significant differences were identified by One-way ANOVA. The symbol “*” indicates a significant reduction (P < 0.05), compared to control, which was transfected with pGL-3 Basic alone.