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. 2015 Jul 9;10(7):e0131711. doi: 10.1371/journal.pone.0131711

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© 2015 Sun et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Nuclear proteins extracted from the gills of P. fucata were incubated with oligonucleotide probes labelled with DIG-ddNTP to perform EMSAs. Lane 1: labelled DNA probes, nuclear protein and antibody of Pf-Rel. Lane 2: labelled DNA probes and nuclear protein. Lane 3: labelled DNA probes. Lane 4: unlabelled DNA probes and nuclear protein. Lane 5: positive control. Band a: free DNA. Band b: DNA and Pf-Rel. Band c: DNA, Pf-Rel and antibody of Pf-Rel. Band d: DNA, Pf-Rel and other interactive nuclear protein. Band e: positive control band. Compared to Lane 2, there is a super shift after Pf-Rel antibody was added in Lane 1, suggesting that Pf-Rel present in the total nuclear protein extracts is capable of binding to the Nacrein promoter probes.