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. 2015 May 11;125(6):2473–2483. doi: 10.1172/JCI74159

Figure 4. Snail1 is sufficient to disrupt brain endothelial tight junctions.

Figure 4

Overexpression of Snail1 resulted in a loss of tight junction proteins (A–D). Control cell line shows the expected cobblestone pattern, and overexpression of Snail1 resulted in a loss of tight junction staining (scale bars: 20 μm) (E). Snail1 overexpression increased hBMEC permeability as assessed by Evans Blue dye migration after induction with doxycycline; dye was added to the upper chamber and quantified colorimetrically in the lower chamber (OD600) (F). Experiments were performed at least 3 times in triplicate; error bars represent the SEM of at least 3 biological replicates (F), or the SD for protein analysis of a representative experiment (B–D). Student’s t test was used to determine significance. *P < 0.05; **P < 0.01; ***P < 0.001.