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. 2015 May 11;125(6):2399–2412. doi: 10.1172/JCI80467

Figure 5. MIF promotes glucose uptake and ATP generation in renal epithelial cells and tissues.

Figure 5

(A) Knockdown of MIF with siRNA or inhibition of MIF with ISO-1 decreased the uptake of glucose in Pkd1 mutant MEK cells compared with untreated Pkd1 mutant MEK cells. n = 3, P < 0.001; ANOVA post hoc test. (B) Knockdown of MIF with siRNA or inhibition of MIF with ISO-1 decreased the levels of intracellular ATP in Pkd1 mutant MEK cells compared with untreated Pkd1 mutant MEK cells. n = 3, P < 0.001; ANOVA post hoc test. (C and D) Treatment with ISO-1 decreased the levels of intracellular ATP in kidneys from Pkd1fl/fl Ksp-Cre mice at PN7 (C) and from Pkd1fl/fl Pkhd1-Cre mice at PN25 (D). n = 5, P < 0.001; ANOVA post hoc test. (E) qRT-PCR analysis of the expression of Hk1, Ldha, and Pkm2 mRNA in PN25 kidneys from Pkd1fl/fl Pkhd1-Cre mice treated with ISO-1 or DMSO. n = 3, P < 0.05; ANOVA post hoc test. The mRNA levels of these genes were normalized to mRNA levels in WT kidneys, which is set as 1.