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. 2015 May 11;125(6):2399–2412. doi: 10.1172/JCI80467

Figure 6. MIF regulates renal epithelial cell proliferation through activation of ERK, mTOR, and Rb/E2F signaling pathways.

Figure 6

(A and B) Western blot analysis of the expression of ERK and phospho-ERK, as well as AMPK and phospho-AMPK, from whole-cell lysates of Pkd1 null MEK (Null-MEK) cells (A) and Pkd1 mutant PN24 cells (B) induced with MIF (10 ng/ml) at indicated time points. (C) Western blot analysis of the expression of ERK and phospho-ERK, AMPK and phospho-AMPK, S6 and phospho-S6, and Rb and phospho-Rb from whole-cell lysates of Pkd1 mutant PN24 cells treated with ISO-1 (100 μM) at indicated time points. (D) Western blot analysis of the expression of ERK and phospho-ERK, AMPK and phospho-AMPK, S6 and phospho-S6, and Rb and phospho-Rb from whole-cell lysates of Pkd1 mutant PN24 cells transfected with MIF siRNA or control siRNA. (E and F) Western blot analysis of the expression of ERK and phospho-ERK, AMPK and phospho-AMPK, S6 and phospho-S6, and Rb and phospho-Rb in kidneys from 3 different Pkd1fl/fl Ksp-Cre mice (E) and Pkd1fl/fl Pkhd1-Cre mice (F) treated with DMSO or ISO-1.