Figure 1.
HIV Gag-Pol constructs. GPV-Δ, GPV-RRE, GPV-1 × CTE and GPV-4 × CTE were cloned into the pcDNA 3.1 vector with a CMV-IE promoter (CMV) and a bovine growth hormone polyadenylation signal (BGH pA). Splice donors (SD) and splice acceptors (SA) are marked. GP-RevInd was a codon-optimized Gag-Pol cloned into the pCI-Neo vector with the CMV-IE promoter (CMV) and the SV40 polyadenylation signal (SV40 pA) (Kotsopoulou et al, 2000). The vector contained an intron 5′ of the Gag-Pol coding region.