Skip to main content
. 2004 Jun 17;23(13):2632–2640. doi: 10.1038/sj.emboj.7600270

Figure 2.

Figure 2

Rev-independent HIV Gag-Pol expression results in efficient Gag processing and budding in murine cells. HeLa (lanes 1 and 2) or 3T3 (lanes 3 and 4) cells were transfected with GPV-RRE+pcRev (lanes 1 and 3) or GP-RevInd+pcDNA 3.1 (lanes 2 and 4) expression vectors and then analyzed at ∼40 h for the presence of Gag proteins in whole-cell lysates and culture media by immunoprecipitation/immunoblot. The p24Gag content in the media from the 3T3 cells was also quantitated by ELISA, and GPV-RRE-transfected cells (lane 3) produced 0.88 ng/ml and the GP-RevInd-transfected cells (lane 4) produced 12 ng/ml.