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. Author manuscript; available in PMC: 2015 Jul 9.
Published in final edited form as: J Cell Sci. 2008 Feb 19;121(0 6):753–761. doi: 10.1242/jcs.017681

Fig. 3.

Fig. 3

Accumulation of GAT1-SSS in punctate structures requires the COPII-dependent ER-export machinery. (A) HEK293 cells were transfected with a plasmid encoding YFP-tagged GAT1-SSS. Cells were treated overnight with H89 (100 μM). Images were acquired 24 hours after transfection. (B,C) HEK293 cells were co-transfected with plasmids coding for CFP-tagged GAT1-SSS (1 μg) and Sar1-T39N (2 μg, B) or YFP-tagged Sec24D-VN (2 μg, C). Images were acquired 24 hours after transfection. (D) HEK293 cells were transfected with a plasmid coding for the double mutant YFP-tagged GAT1-RL/AS-SSS and images were captured 24 hours after transfection. (E) HEK293 cells were co-transfected with plasmids encoding SAR1A-T39N (2 μg) and either YFP-tagged wild-type GAT1 (wt; 1 μg) or YFP-tagged GAT1-SSS (sss; 1 μg). FRAP was recorded as outlined in the Materials and Methods. Data represent mean half-lives in seconds of fluorescence recovery from five independent experiments; error bars indicate s.e.m.