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Localization of GAT1-SSS in hippocampal neurons. Hippocampal neurons were isolated from foetal rat brains (E18) and transfected after 7 days in culture using Lipofectamine 2000 with a plasmid encoding YFP-tagged GAT1-SSS. The transporter was visualized by confocal fluorescence microscopy 48 hours after transfection.
