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. 2004 Jun 17;23(13):2520–2530. doi: 10.1038/sj.emboj.7600271

Figure 8.

Figure 8

Mmr1p localization in myo2 mutants. (A) myo2-573 MMR1HA cells (upper panel) and myo2-338 MMR1HA cells (lower panel), carrying a plasmid for mitochondria-targeted GFP, were grown to mid-log phase in SC, fixed, and stained using anti-HA antibodies visualized with Alexa 546. (a) Mitochondria (green); (b) Mmr1pHA (red); and (c) merged. Scale bar: 5 μm. Rare myo2-573 cells, carrying mitochondria in the bud, were selected for the figure, to show that Mmr1p still delocalized even when mitochondria were distributed to the bud. (B) pDsRed-Mmr1-270C was introduced into myo2-338-GFP cells (a–c) and myo2-573-GFP cells (d–f), where MYO2 was replaced with the GFP-tagged version of myo2-338 and myo2-573, respectively (Itoh et al, 2002). Cells were cultured in SC, shifted to SCRGD, harvested after 12 h from the shift, and fixed. (a, d) Ds-Red signal; (b, e) GFP signal; and (c, f) phase contrast.