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. 2014 Apr 30;34(18):6344–6351. doi: 10.1523/JNEUROSCI.2991-13.2014

Figure 6.

Figure 6.

Dominant-negative SEC24C precludes delivery of SERT into the axonal compartment of rat dorsal raphe neurons. Rat dorsal raphe neurons were transfected with plasmids coding for CFP–SEC24C (A), CFP–SEC24C–VN (B), or YFP–SEC24D–VN (C) using Lipofectamine2000. After 48 h, the neurons were fixed and stained for endogenous SERT (H-45) and MAP-2. The immunoreactivity was visualized with Alexa Fluor 568-, Alexa Fluor 488-, or Alexa Fluor 633-conjugated secondary antibodies. In C, the small white arrow highlights a glial cell transfected with YFP–SEC24D–VN only. Images were captured by confocal microscopy. During expression of CFP–SEC24C–VN, SERT was confined to the MAP-2-positive compartment in 18 of 18 examined neurons; in the presence of dominant-negative SEC24D, SERT reached the MAP-2-negative compartment in 14 of 14 examined neurons. Data are from four independent experiments (i.e., 4 individual preparations of rat dorsal raphe neurons that were independently transfected in parallel with plasmids encoding CFP–SEC24C–VN or YFP–SEC24D–VN). Scale bars, 20 μm.