. Author manuscript; available in PMC: 2015 Nov 1.

Published in final edited form as: J Struct Biol. 2014 Sep 30;188(2):183–187. doi: 10.1016/j.jsb.2014.09.008

Table 1.

Comparisons of the data acquisition conditions between the T20S dataset described in this manuscript and the one reported by Li et al (Li et al., 2013b).

	TF20	Polara
C2 aperture size (µm)	50	30
Size of area illuminated (µm)	~1.2	~3
Beam intensity (counts/pixel/sec)	11.2	8
Frame rate (frame per second)	5	5
Number of frames per movie	25	25
Total exposure (e−/Å²)	38	35
Number of micrographs	166	553
Total number of particles used in the final map	21,818	126,729
Defocus range (µm)	0.725–3.3 (200kV)	0.8–1.9 (300kV)
Final resolution (Å)	4.2	3.3