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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2015 May 14;14(7):1728–1737. doi: 10.1158/1535-7163.MCT-14-0672

Figure 1. Aromatase inhibitor-resistant cells generated increased levels of ROS and expressed lower levels of INrf2, higher levels of Nrf2 and Nrf2 downstream gene expression.

Figure 1

(A) Cellular reactive oxygen species (ROS) mediated fluorescence in letrozole-sensitive (MCF-7Ca) and letrozole-resistant LTLTCa) cells. Cells were incubated with 2′7′-dichlorofluorescein diacetate (DCFDA) and cellular ROS–mediated fluorescence intensity was recorded using microplate reader. (B/D/F) MCF-7Ca and LTLTCa Cells were lysed and analyzed by Western blot. C) Cytosolic and nuclear fractions from MCF-7Ca and LTLTCa cells were immunobloted. (E) Total and reduced glutathione in MCF-7CA and LTLTCa cells was determined spectrophotometrically at 570 nm. (F) Western blot analysis of the relative levels of Nrf2, INrf2 (Keap1) and Nrf2 target genes anastrozole-sensitive AC1 and Aanstrozole-resistant AnaR cells.