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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2015 May 14;14(7):1728–1737. doi: 10.1158/1535-7163.MCT-14-0672

Figure 4. Tumor initiating cells (TIC) isolated from LTLTCa expressed lower levels of INrf2 and higher levels of Nrf2.

Figure 4

LTLTCa cells were subjected to aldefluor staining (Aldefluor staining kit, Stem Cell Technologies) and the cells expressing ALDH were further gated into ALDH-high and ALDH-low cells. Cells were sorted as ALDH High (TIC) and ALDH Low (non-TIC) fractions. MCF-7Ca and LTLTCa cells also received the same treatment as LTLTCa-low ALDH and LTLTCa-high ALDH cells. MCF-7Ca and LTLTCa cells were used as control cells. The cells were lysed and total cell lysate was immumoblotted with indicated antibodies. *Control live cells were sorted by propidium iodide exclusion.