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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2015 May 14;14(7):1728–1737. doi: 10.1158/1535-7163.MCT-14-0672

Figure 5. Increased Nrf2 in LTLTCa cells is directly associated with higher ALDH. (A–B) LTLTCa cells containing higher Nrf2 showed increased ALDH.

Figure 5

(A) Western analysis. MCF-7Ca and LTLTCa cells were immunoblotted for Nrf2 and β-actin. (B) ALDH measurement. MCF-7Ca and LTLTCa cells were stained with Aldefluor in absence and presence of ALDH inhibitor DEAB (diethylaminobenzaldehyde) and the percentage of cells expressing ALDH was determined. (C-D) Nrf2-Knocked down LTLTCa cells expressed lower levels of ALDH. Two clones of LTLTCa cells expressing Nrf2shRNA were selected. (A) LTLTCa-V and LTLTCa-Nrf2KD cells were lysed and immunoblotted with Nrf2 to confirm reduced levels of Nrf2. (B) All the cells were subjected to Aldefluor staining and the percentage of cells expressing ALDH was determined by comparing the same sample with and without the ALDH inhibitor DEAB. LTLTCa-Nrf2KD cells contained lower percentage of cells expressing ALDH. Data was acquired using FACS CANTO.