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. Author manuscript; available in PMC: 2016 Jul 7.
Published in final edited form as: Cell Rep. 2015 Jun 25;12(1):49–57. doi: 10.1016/j.celrep.2015.06.010

Figure 2. Maternal dazap2 is required for maintenance of germ granules in PGCs.

Figure 2

A) Endogenous Vasa and Ziwi protein localization in dazap2ae13 and MZdazap2 mutant PGCs at 30hpf. Both Vasa and Ziwi protein fail to localize to perinuclear germ granules in Mdazap2 mutants. Scale bar=8μm. B) Quantification of PGC germ granules of each genotype indicates %PGCs with granules greater than or less than 1 μm. Quantification of Vasa+ and Ziwi+ granules from heterozygous intercrosses (het inX) and MZdazap2 embryos represent n>90 PGCs from 10 embryos for each condition. C) Germ granule morphology at 4 and 10hpf using Vasa protein to label germ granules. Scale bar=15μm. Yellow dotted line marks PGC. D) Quantification of germ granule size shows variation at 4 and 10 hpf. All p-values>0.05. E) Live imaging of PGCs at 11 and 30 hpf expressing CLIP170-GFP reveals microtubules of WT and MZdazap2 mutants. Scale bar=8μm. F) Live imaging of PGCs at 30 hpf expressing Dynll2b-GFP reveals the localization of Dynein motor protein complexes in WT and MZdazap2 PGCs. Dashed yellow line outlines the PGC and “n” denotes the PGC nucleus. G) Vasa protein at 14dpf. H) Quantification of Vasa positive PGCs at 14dpf. p value=0.0566.