All Fz1/2 chimeras shown are Myc-tagged (the tag being inserted right after the CRD of Fz1 or Fz2; see Materials and Methods; Boutros et al. 2000). The respective Fz1/2 chimeras, with their schematic structure shown under each photomicrograph, were expressed under dpp-Gal4 (expression domain marked with UAS-EGFP in example in [A]) and analyzed by confocal microscopy xz-sections (perpendicular to the stripe of expression in the wing pouch region).
(A) Subcellular localization of wild-type Fz-Myc (Fz1–1-1, in green; red channel shows coexpressed GFP to mark expressing cells). Single-channel black-and-white staining of Fz-Myc is shown on right.
(B–F) Anti-Myc staining of different Fz1/2 chimeras: (B) Fz1–2-2, (C) Fz1–1-2, (D) Fz2–1-1, (E) Fz1–2-1, and (F) Fz2–2-1.
(G) Fz2–2-2. Note the correlation of apical Fz localization with the presence of the Fz1 C-tail.