Vemurafenib induces significant loss of HKII in both sensitive and intrinsically resistant lines. A, 3μM Vemurafenib induces a decrease in overall hexokinase activity per cell after 72 hours in sensitive cell lines B, Vemurafenib (+) induces near complete loss of HKII in sensitive but not C, acquired resistant or non-BRAF cell lines at 72 hours relative to DMSO (−). D, Intrinsically resistant cell lines show a decrease in HKII comparable to sensitive cell lines. E, Sensitive and intrinsically resistant lines show a significant increase in HKI with Vemurafenib treatment by 72 hours that is not observed in acquired resistant or non-BRAF mutant lines, F.