Figure 6.

Glucose uptake is greatly affected by changes cell volume. A, Decreasing solution osmolarity while incubating with 2-NBDG causes both an increase in cell volume and B, 2-NBDG uptake that is osmolarity-dependent. The isotonic condition has a total concentration of 289 mOsm/L, while the weakly and strongly hypoosmotic conditions have concentrations of 144.5 mOsm/L and 72.25 mOsm/L respectively. C, Incubation of 3μM vemurafenib-treated cells in hypoosmotic 2-NBDG solution restored glucose uptake to the same level as isotonic 2-NBDG control at 217 mOsm/L. D. Inhibition of protein translation with 50 μg/mL cycloheximide induces a decrease in both cell volume and E, glucose uptake at 24 hours with F, a relationship similar to 3μM vemurafenib (r² = 0.8153, p = 0.097). G, Inhibition of translation with cycloheximide produces comparable loss of HKII to Vemurafenib by 24h. H, ³⁵S methionine labeling shows that incubation of vemurafenib sensitive but not resistant lines for 72h with 3μM reduced total new protein synthesis, *, p < 0.05; **, p < 0.01; ***, p < 0.001.