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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cancer Discov. 2015 Apr 16;5(7):740–751. doi: 10.1158/2159-8290.CD-14-1347

Figure 2. Loss of Inpp4b in Pten+/− mice leads to follicular thyroid carcinoma.

Figure 2

A. Kaplan-Meier survival curve of Pten+/−, Pten+/−Inpp4b+/− and Pten+/−Inpp4b−/− mice. B. Top panel: gross anatomy of representative thyroids from mice of the indicated genotypes. White arrows point to the location of the thyroid. Bottom panel: dissected thyroids from mice of the indicated genotypes; Scale bar, 2mm. C. Left panel: H&E staining of a follicular thyroid carcinoma (FTC)-like thyroid tumor which is thinly encapsulated with microfollicular architecture. Right panel: H&E staining of a follicular variant of papillary thyroid carcinoma (FV-PTC)-like thyroid tumor. Middle and bottom panels highlight nuclear atypia characteristic of FV-PTC, including intranuclear pseudoinclusions (arrow). Scale bars, top and middle panels: 100μm, bottom panel: 20 μm, D. Top panel: H&E staining of thyroid tumor and lung metastases from Pten+/−Inpp4b−/− mice; middle & bottom panel: thyroglobulin and p-Akt (Ser473) staining. Scale bar, 100μm. Insets show thyroid cancer cells. Blue arrows point to the location of the metastases in the lungs.