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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cancer Discov. 2015 Apr 16;5(7):740–751. doi: 10.1158/2159-8290.CD-14-1347

Figure 5. Knockdown of INPP4B in thyroid cancer cells provides an advantage for anchorage independent growth.

Figure 5

A. RT-qPCR analysis of INPP4B upon shRNA-mediated knockdown. B. Western blot analysis of INPP4B levels in TPC1 and 8505C cell lines after shRNA-mediated knockdown. C. Proliferation of TPC1 and 8505C cell lines infected with either a non-targeting shRNA or a shRNA that targets INPP4B. Cells were cultured in complete media (10% serum), stained with crystal violet, and lysed. Absorbance was measured at OD595nm. D-E. Soft agar colony formation assay of cells infected with either a non-targeting shRNA or an shRNA which targets INPP4B. Cells were grown in soft agar for 2 weeks before being photographed (D) and the number of colonies was then counted (E).