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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Hum Genet. 2015 May 31;134(8):905–916. doi: 10.1007/s00439-015-1570-5

Figure 2. SRGAP1 is coexpressed with ROBO2 in cap mesenchyme in rat kidney at E16.5 and P0.

Figure 2

Immunofluorescence microscopy of sections of rat kidneys at E16.5 (A and B) and at P0 (C and D).

(A–B) ROBO2 (green) is expressed in SIX2-positive (blue-green) cap mesenchyme and developing glomeruli. SRGAP1 (red) colocalizes with ROBO2 in cap mesenchyme.

(B) Enlargement from (A). Note that the overlap of ROBO2 and SRGAP1 (yellow) in cap mesenchyme is most prominent at the ureteric tip-facing pole of the cap mesenchyme (arrows) indicating active ROBO2-SRGAP1 signaling at this site.

(C–D) ROBO2 is expressed in SIX2-positive cap mesenchyme (arrow-heads) and in different stages of development of glomerular podocytes (C, comma-shaped body; CL, capillary loop stage glomerulus; G, mature-appearing glomerulus).

(C) Overview of renal cortex showing different stages of embryonic nephron development. The nephrogenic zone is marked by SIX2-positive cap mesenchyme directly underneath the renal capsule (arrow-heads). Different stages of glomerular development on a gradient from early (C, CL) to late (G) express ROBO2 and SRGAP1.

(D) SRGAP1 (red) is coexpressed with ROBO2 (green) in cap mesenchyme (arrow-heads) and highly expressed in developing podocytes in capillary loop stages glomeruli (arrows).

Scale bar: 50µm.