Skip to main content
. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Hum Genet. 2015 May 31;134(8):905–916. doi: 10.1007/s00439-015-1570-5

Figure 4. SLIT2 mutations identified in CAKUT patients compromise its inhibition of SVZa neuronal migration.

Figure 4

(A) Representative DIC images show SVZa neuronal migration after 24 hours in the presence of SLIT2 wild-type (WT) or SLIT2 mutants (A98T, S566N, K904N) conditioned media. Media from mock transfected HEK293T cells were used as a negative control. Note that wild-type SLIT2 inhibits neuronal migration whereas the SLIT2 mutants show a diminished inhibition. Dot circles in processed image column represent the areas where the number of migrated cells out of SVZa explants and migration distance were quantified (see also Supplementary Figure 6 online).

(B) Quantification of migrated SVZa cell counts in (A). Note wild-type SLIT2 has less migrated cells out of SVZa explants. SLIT2 mutants A98T, S566N, and K904N show a diminished inhibitory effect on migrated cell counts.

(C) Quantification of average SVZa cell migration distances in (A). Note wild-type SLIT2 inhibits migration of SVZa cells. SLIT2 mutants A98T, S566N, and K904N show a reduced inhibitory effect on migration indicating partial loss-of-function.

Scale bar: 100µm.

*, P< 0.05 (Student’s t-test); Error bars indicate one standard deviation (n=3).