Phylogenetic analyses of alphavirus isolates. A midpoint rooted Bayesian Markov chain Monte Carlo phylogeny was constructed based on (A) 810 nt of the E1/3′-NCR genome region for VEEV, (B) a structural polyprotein ORF (26S) for VEEV, (C) 810 nt of the E1/3′-NCR for EEEV, and (D) 993 nt of the E1/3′-NCR for MAYV/UNAV. Venezuelan sequences generated in this study are highlighted in bold. Tip labels include the year of isolation, strain name, and country of isolation. Clade credibility values over 90% are shown adjacent to their corresponding nodes. The scale bar represents the percentage of nucleotide sequence divergence.