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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Anal Biochem. 2015 May 8;484:1–3. doi: 10.1016/j.ab.2015.05.002

Figure 1. RNA degradation during purification from PEG hydrogels.

Figure 1

Total RNA was purified using a combined TRIzol® /column purification protocol. MDA-MB-231 cells were embedded in PEG hydrogels (1 million cells/mL) (B) control MDA-MB-231 cells plated on 60 mm tissue culture plates were lysed with 1 mL TRIzol (Life Technologies) and homogenized using pellet pestle (Kimble-Kontes, Vineland, NJ) followed by centrifugation. The aqueous phase was collected and total RNA was purified with RNeasy plus mini kit (Qiagen) according to the manufacturer’s protocol. RNA integrity was analyzed using RNA 6000 Pico Kit on Agilent Bioanalyzer.