Table 1.
Radioactivity concentration of the brain after intravenous injection of [3H]hydromethidine to tMCAO mice treated with saline and DMTU
| Brain region | CTX-1 (Bq/mg) | CTX-2 (Bq/mg) | CTX-3 (Bq/mg) | STR-1 (Bq/mg) | STR-2 (Bq/mg) | HIP (Bq/mg) | TH (Bq/mg) | ||
|---|---|---|---|---|---|---|---|---|---|
| Control | Right | 30.5 ± 2.7 | 30.7 ± 3.0 | 29.1 ± 1.3 | 30.5 ± 2.3 | 31.2 ± 2.5 | 30.6 ± 2.7 | 35.3 ± 3.6 | |
| Left | 30.2 ± 2.7 | 31.0 ± 2.8 | 29.6 ± 2.5 | 30.3 ± 2.5 | 31.2 ± 2.7 | 30.2 ± 3.7 | 34.9 ± 3.9 | ||
| tMCAO + Saline | 1 h | Right (C) | 25.9 ± 2.0 | 26.1 ± 1.2 | 26.3 ± 2.0 | 26.5 ± 1.4 | 27.0 ± 1.5 | 29.9 ± 2.1 | 29.8 ± 2.5 |
| Left (I) | 28.0 ± 1.6 | 29.1 ± 1.1 | 33.4 ± 5.0 | 41.9 ± 7.2 | 64.4 ± 17.2 | 34.8 ± 6.0 | 32.7 ± 4.6 | ||
| 2 h | Right (C) | 37.9 ± 9.1 | 37.6 ± 8.9 | 37.0 ± 8.3 | 39.1 ± 9.0 | 39.3 ± 9.5 | 43.6 ± 12.0 | 42.2 ± 8.8 | |
| Left (I) | 36.4 ± 6.5 | 36.9 ± 6.8 | 37.0 ± 7.3 | 44.9 ± 23.8 | 50.4 ± 31.4 | 49.1 ± 9.5 | 40.7 ± 5.5 | ||
| 5 h | Right (C) | 21.4 ± 2.6 | 21.3 ± 2.2 | 20.9 ± 2.4 | 22.1 ± 2.9 | 22.7 ± 2.9 | 23.8 ± 2.8 | 24.4 ± 2.8* | |
| Left (I) | 31.1 ± 7.2 | 30.0 ± 6.5 | 28.7 ± 10.0 | 62.5 ± 18.5* | 68.4 ± 19.8* | 49.7 ± 20.2 | 34.2 ± 12.4 | ||
| 7 h | Right (C) | 36.2 ± 5.5 | 36.0 ± 5.5 | 35.6 ± 5.4 | 37.7 ± 5.8 | 38.1 ± 5.7 | 40.3 ± 7.5 | 41.6 ± 4.6 | |
| Left (I) | 42.6 ± 11.0 | 44.7 ± 14.1* | 48.3 ± 13.5** | 64.7 ± 15.5** | 72.7 ± 18.2** | 62.2 ± 21.2** | 49.5 ± 10.6* | ||
| tMCAO + DMTU | 1 h | Right (C) | 34.6 ± 7.7 | 34.7 ± 7.1 | 33.5 ± 5.8 | 35.2 ± 6.6 | 36.1 ± 7.2 | 41.4 ± 8.3 | 38.3 ± 7.6 |
| Left (I) | 33.1 ± 6.8 | 33.5 ± 5.8 | 35.2 ± 7.4 | 34.0 ± 9.8 | 42.8 ± 20.6 | 43.7 ± 15.1 | 38.2 ± 6.9 | ||
| 5 h | Right (C) | 38.6 ± 4.9 | 38.6 ± 4.7 | 38.1 ± 4.5 | 40.8 ± 5.9 | 49.2 ± 7.3** | 44.3 ± 5.8 | ||
| Left (I) | 46.4 ± 13.5* | 46.5 ± 10.0* | 46.0 ± 11.6* | 62.5 ± 19.9* | 69.1 ± 18.8* | 51.1 ± 12.0 | 41.3 ± 4.2 | ||
| 7 h | Right (C) | 62.4 ± 10.5** | 61.6 ± 10.0** | 61.2 ± 9.5** | 64.8 ± 11.4** | 64.4 ± 11.6** | 78.3 ± 14.0** | 66.9 ± 10.9** | |
| Left (I) | 63.8 ± 10.7** | 64.3 ± 10.1** | 64.2 ± 8.3** | 79.0 ± 19.5** | 80.6 ± 19.6** | 76.7 ± 15.1** | 65.8 ± 11.2** | ||
[3H]Hydromethidine was intravenously injected into mice treated with saline or DMTU (200 mg/kg, i.p.) 30 min before tMCAO (90 min). Coronal sections at the level of the striatum and hippocampus were prepared at 1, 2, 5, and 7 h after ischemia/reperfusion of MCA in mice. Data are expressed as mean ± SD (Bq/mg, n = 5–8 for each time point)
C contralateral, I ipsilateral
*P < 0.05, **P < 0.01, as compared with control mice