Figure 2.

Experimental design for studying the Arabidopsis response to Hyaloperonospora arabidopsidis (Hpa). Protoplasts were generated from populations of seedlings containing the Pro_DMR6::GFP transgene, with or without Hpa Noks1 infection, and sorted using Fluorescence Activated Cell Sorting to yield three types of cell population: (A) uninfected control cells, (B) GFP-positive cells from infected plants (Hpa-proximal cells), and (C) GFP-negative cells from infected plants (Hpa-distal cells). Whole genome expression profiling was performed from isolated cells at two time points (5 and 7 d.p.i), each with three biological replicates.