FIGURE 1.

Topology and sequence of Cx32 shown together with one subunit of the modeled gap junction channel. A, membrane topology of Cx32 showing four transmembrane helices, cytoplasmic N and C termini, and two extracellular loops. Transmembrane helices are colored as follows: TM1 (blue), TM2 (green), TM3 (yellow), and TM4 (red). B, alignment of rCx26 and hCx32 obtained using HHpred, which was used to create the model of rCx32. C, one subunit of the modeled Cx32 channel viewed from the center of the pore. The model was created using MODELLER.