FIGURE 2.

Results of tryptophan substitution summarized in histogram format. Domains sketched adjacent to each graph show the orientation of TM domains (NH₂, cytoplasmic N terminus; EL1, first extracellular loop; CL, cytoplasmic loop; EL2, second extracellular loop; COOH, C terminus). Numbers beside each bar indicate the number of pairs with measurable conductance along with the number of pairs tested (e.g. number functional/number tested). Each bar represents the mean junctional conductance between oocytes paired heterotypically (Cx32/mutant) and normalized to Cx32/Cx32 pairs of the same batch on the same day. Conductance was reduced (blue), abolished (red), or unaffected (black). NF, nonfunctional, *, statistically different from wild type at p ≤ 0.05 as determined by Student's t test.