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Binding specificity of Xyn10C. a, SDS-PAGE analysis of binding to insoluble polysaccharides. Proteins (P) were mixed with Avicel or with the insoluble fraction of oat spelt xylan for 1 h; unbound (U) and bound (B) fractions were separated by centrifugation; pellets were washed three times (W). b, nondenaturing polyacrylamide gels containing no ligand (control) or soluble polysaccharides. Lane 1, BSA; lane 2, Xyn10C; lane 3, Xyn10C-CD; lane 4, Xyn10C-XBD.
