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Subcellular localization of human and rat UPF0586 protein in HeLa cells visualized by confocal microscopy. HeLa cells were transiently transfected with plasmids encoding human UPF0586-EGFP (A) or rat UPF0586-EGFP (B). Localization of chimeric proteins was determined by the direct fluorescence of EGFP in living cells, while nuclei were visualized by staining with Hoechst 33342. Representative images obtained with a confocal laser-scanning microscope are shown.
