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SDS-PAGE analysis of purified recombinant UPF0586 proteins. Rat (rUPF0586), human (hUPF0586), chicken (chUPF0586), and yeast (yUPF0586) proteins were purified to homogeneity by affinity chromatography on nickel-Sepharose (HisTrap HP), as described under “Experimental Procedures.” For the SDS-PAGE analysis, 10 μl of sample derived from each fraction was loaded onto a 10% gel and electrophoresed, and the resulting gel was then stained with silver (11).
