FIGURE 4.
Amino acid sequence alignment of rat UPF0586 protein with its yeast, chicken, and human orthologues. Sequences were obtained with following GenBankTM accession numbers: yeast (S. cerevisiae, NP_014307.1), chicken (G. gallus, XP_003643080.1), rat (R. norvegicus, NP_001020145.1), and human (H. sapiens, NP_689633.1). All shown sequences have been confirmed by PCR amplification of the cDNA and sequencing. The percentage amino acid identities with rat UPF0586 protein are given at the top right. The highly conserved domain of unknown function (N-2227) is labeled above the alignment, whereas amino acids residues interacting with SAM are indicated by asterisks, as predicted by I-TASSER (29). The peptides identified by mass spectrometry in the protein purified from rat leg muscle are underlined in the rat sequence. The level of residue conservation is indicated as 100% (black background), 70% and more (dark gray background), and 50% and more (light gray background).