TABLE 2.

Purification of carnosine N-methyltransferase from rat skeletal muscles

Fraction	Volume	Total protein	Total activity	Specific activity	Purification	Yield
	ml	mg	pmol min−1	pmol min−1 mg−1	-fold	%
20,000 × g supernatant	400	12,466	1536	0.123	1	100
DEAE-Sepharose	39	207	1296	6.26	51	84.4
Q Sepharose	15	60	614	10.23	83	40.0
Superdex 200	10	22	415	18.86	153	27.0
HiScreen Bluea	2	0.07	22.6	322.86	2625	1.5

^a The data represent mean values for the two most purified fractions (fractions 73 and 74).