TABLE 5.

Kinetic properties of rat, human, chicken, and yeast UPF0586 proteins

Kinetic properties were determined with the use of purified recombinant C-terminal His₆-tagged UPF0586 proteins. Determinations for SAM were performed with enzyme preparations (0.20–0.62 μg protein) that were incubated for 10 min at either 30 °C (yeast) or 40 °C (vertebrates) in the reaction mixture containing 40 mm carnosine and variable concentrations of [¹H + ³H] SAM (∼800 × 10³ cpm), whereas the measurements for carnosine were done in the presence of non-saturating 1 μm concentration of [¹H + ³H] SAM (100 pmol, ∼600 × 10³ cpm). Values are the means of three separate experiments. The S.E. value is also given.