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. 2015 May 26;290(28):17239–17249. doi: 10.1074/jbc.M115.655605

FIGURE 2.

FIGURE 2.

SCP3 is essential for Xenopus embryogenesis. A, MO test in Xenopus embryos. Two-cell stage embryos were coinjected with 40 ng of std MO or scp3 MOs and mRNA coding for X. laevis SCP3-FLAG with the indicated doses. At stage 10.5, the embryos were harvested for Western blotting analysis. B, std MO or scp3 MOs was injected into 1-cell stage embryos with the indicated doses. C, 2-cell stage embryos were injected with 40 ng of std MO or scp3 MO-1 with or without 750 pg (left) or 200 pg (right) of X. laevis scp3 mRNA as indicated. D, uninjected oocytes and scp3 MO-1-injected oocytes were processed by the host transfer technique and allowed to develop until the indicated stages.