FIGURE 6.

Drosophila CBP co-activates Bicoid-regulated transcriptional activation of the gt reporter without a preference for a TATA box or a DPE motif. A, CBP and Bicoid occupancy at the gt locus in WT embryos. ChIP-sequencing peaks for CBP (73) and ChIP-chip peaks for Bicoid (82) in 2–4-h old WT embryos are shown for the gt locus. Occupancy is plotted as log₂ -fold enrichment over input. B, Drosophila S2R+ cells were co-transfected with gt reporter constructs that contain the gt enhancer and promoter sequences from −2031 to +40 relative to the +1 start site and are identical except for an mTATA or mDPE motif as well as an expression vector for Bicoid and/or dCBP. To normalize for variations in transfection efficiency, cells were co-transfected with a Pol III-Renilla luciferase control plasmid and assayed for Dual-Luciferase activity. The activities are reported relative to the activities of the promoters in the absence of co-transfected Bicoid or dCBP expression plasmids, which were defined to be 1. The graph represents an average of three independent experiments. Error bars represent S.E.