FIGURE 7.

Drosophila CBP can convey preferential transcriptional activation of the ftz reporter to both the N terminus and the C terminus of Caudal. A, schematic representation of the full-length FLAG-Caudal protein and the mini-Caudal proteins containing the N terminus and homeodomain of Caudal (N-term. + HD; aa 2–334) or the homeodomain and C terminus of Caudal (HD + C-term.; aa 270–427). The blue box at the N terminus of the Caudal expression constructs denotes a FLAG tag. B, Drosophila S2R+ cells were co-transfected with ftz reporter constructs containing either an mTATA or a mDPE motif as well as plasmids encoding dCBP, FLAG-tagged full-length-Caudal, or a mini-Caudal protein as depicted. To normalize for variations in transfection efficiency, cells were co-transfected with a Pol III-Renilla luciferase control plasmid and assayed for Dual-Luciferase activity. The activities are reported relative to the activities of the promoters in the absence of co-transfected Caudal or dCBP expression plasmids, which were defined to be 1. The graph represents an average of three independent experiments. Error bars represent S.E. ns, p > 0.05; ***, p ≤ 0.001.