TABLE 1.

Gene-specific primer sequences and RT-PCR conditions

Gene	PCR sequence (5′–3′)	Annealing temperature	Cycle	Product size
		°C		bp
Th
Forward	TCAAGACTGACTCACAGCAACCCC	60	25	412
Reverse	CTTTGTCCTGAACCGTGGTGGTAG
Gapdh
Forward	GGCATTGCTCTCATTGACAA	60	23	165
Reverse	AGGGCCTCTCTCTTGCTCTC