TABLE 3.
Primary antibody information
| Antibody | Dilution | Source | Location |
|---|---|---|---|
| Polyclonal antibody (rabbit) | |||
| Nestin #130 (NES) | 1:50 | Dr. R. McKay (NIH) | Bethesda, MD |
| Tyrosine hydroxylase (TH) | 1:5000 | Pel-Freez | Rogers, AR |
| GABA | 1:5000 | Sigma-Aldrich | St. Louis, MO |
| Neuron-specific class III β-tubulin (TUJ1) | 1:5000 | Covance | Richmond, CA |
| Glia fibrillary acidic protein (GFAP) | 1:400 | DAKO | Glostrup, Denmark |
| Potassium inwardly rectifying channel subfamily J, member 6 (GIRK2) | 1:100 | Sigma-Aldrich | St. Louis, MO |
| Green fluorescent protein (GFP) | 1:2000 | Life Technologies | Eugene, OR |
| SOX2 | 1:500 | Chemicon | Temecula, CA |
| PITX3 | 1:200 | Chemicon | Temecula, CA |
| Vesicular monoamine transporter 2 (VMAT2) | 1:500 | Pel-Freez | Rogers, AR |
| NURR1 | 1:500 | Santa Cruz Biotechnology | Dallas, TX |
| Synapsin I | 1:1000 | Sigma-Aldrich | St. Louis, MO |
| Collagen I | 1:500 | Abcam | Cambridge, UK |
| Cleaved caspase-3 | 1:500 | Cell Signaling | Danvers, MA |
| Serotonin | 1:4000 | Sigma-Aldrich | St. Louis, MO |
| Monoclonal antibody (mouse) | |||
| Nestin (NES) | 1:500 | BD Biosciences | Franklin Lakes, NJ |
| TH | 1:2000 | Sigma-Aldrich | St. Louis, MO |
| TUJ1 | 1:2000 | Covance | Richmond, CA |
| CNPase | 1:500 | Sigma-Aldrich | St. Louis, MO |
| Microtubule-associated protein 2 (MAP2) | 1:1000 | Sigma-Aldrich | St. Louis, MO |
| GFAP | 1:500 | MP Biomedicals | Santa Ana, CA |
| Glutamate clone glu4 (GluT) | 1:5000 | Sigma-Aldrich | St. Louis, MO |
| Smooth muscle actin (SMA) | 1:1000 | Sigma-Aldrich | St. Louis, MO |
| KI67 | 1:100 | Novocastra | Newcastle, UK |
| HuC/D | 1:100 | Chemicon | Temecula, CA |
| Other host | |||
| Dopamine transporter (DAT), anti-rat | 1:200 | Abcam | Cambridge, UK |
| FOXA2, anti-goat | 1:500 | Santa Cruz Biotechnology | Dallas, TX |