TABLE 2.
FBBBE fluorescence
Fluorescence in arbitrary units/min when 1.8 × 105 murine neutrophils were incubated for 10 min with agents listed in the left column prior to adding 50 μm FBBBE + PBS or 100 nm DENO and monitoring for 5 min, which was the duration of linear rise in fluorescence readings. The data are mean rates ± S.E., n = 6 replicate experiments performed with fresh neutrophil isolates from different mice for all groups.
Agent | Control | 100 nm DENO |
---|---|---|
PBS | 1.29 ± 0.07 | 3.07 ± 0.15a |
Nox2ds | 1.00 ± 0.17 | 0.97 ± 0.11 |
1400W | 0.87 ± 0.07 | 1.20 ± 0.12 |
Geldanamycin | 1.33 ± 0.17 | 1.40 ± 0.38 |
a p < 0.05 versus control.