TABLE 4.

Protein associations assessed by DTSP cross-linking in sF-actin, G-actin, and Triton-insoluble actin fractions of neutrophils incubated with PBS (control) or 100 nm DENO for 2 min

The values are ratios calculated based on the band densities of the identified protein relative to the actin band density in each sample. In each table, the values are normalized to air-exposed control protein band ratios of individual experiments; thus, values greater than 1.0 reflect increases in protein associations with actin, whereas values less than 1.0 indicate lower protein associations. Where indicated cells were exposed to UV light for 5 min prior to addition of DTSP to cross-link proteins and then fractioned based on Triton solubility (see “Experimental Procedures”). The data are the means ± S.E. (n = 4 for all samples).

	VASP/actin	FAK/actin	iNOS/actin	PDI/actin
sF-actin
DENO	3.85 ± 1.02a	2.08 ± 0.17a	2.31 ± 0.17a	5.07 ± 1.25a
PBS + UV	1.37 ± 0.6	1.14 ± 0.24	1.10 ± 0.23	1.51 ± 0.33
DENO +UV	1.13 ± 0.19	0.97 ± 0.19	1.21 ± 0.47	1.26 ± 0.24
G-actin
DENO	0.16 ± 0.09a	0.15 ± 0.08a	0.39 ± 0.16a	0.21 + 0.10a
PBS + UV	0.82 ± 0.22	0.81 ± 0.12	0.73 ± 0.12	0.74 ± 0.13
DENO +UV	1.21 ± 0.32	1.02 ± 0.18	0.65 ± 0.08	0.81 ± 0.25
Triton-insoluble
DENO	0.14 ± 0.12a	0.22 ± 0.08a	0.51 ± 0.13a	0.17 ± 0.08a
PBS + UV	1.06 ± 0.18	0.93 ± 0.07	0.93 ± 0.08	1.07 ± 0.14
DENO +UV	0.88 ± 0.16	0.62 ± 0.11	0.87 ± 0.07	0.95 ± 0.16

^a Values significantly different from normalized air-exposed control values (p < 0.05, ANOVA).