TABLE 3.
Number of channel types determined from single channel measurements
Number of experimentally determined WT, heterotetrameric, and homotetrameric mutant (mutant) channels at indicated expression vector ratios using 7 μg of total DNA/dish. In parentheses is the number of channels assuming uniform distribution of subunits in the channel complexes and WT and mutant subunits at immunoblot ratios shown in Table I.
| RyR1 expression vector ratio | RyR1 channel types |
||
|---|---|---|---|
| WT | Heterotetrameric | Mutant | |
| WT:G4934V | |||
| 3:1 | 9 (10.7) | 6 (16.3) | 12 (0.03) |
| 1:1 | 0 (1.8) | 5 (15.5) | 13 (0.7) |
| 1:3 | 1 (0.2) | 11 (20.3) | 15 (6.5) |
| WT:G4941I | |||
| 3:1 | 8 (11.3) | 10 (14.6) | 8 (0.03) |
| 1:1 | 9 (2.7) | 4 (18.7) | 9 (0.5) |
| 1:3 | 4 (0.2) | 0 (15.6) | 16 (4.2) |