TABLE 1.
Comparison of the changes in RyR2 channel gating in response to Zn2+
The table shows the open and closed lifetime parameters in the presence of 10 μm cytosolic Ca2+ and following the addition of Zn2+ (100 pm to 100 μm) to the cytosolic face of the channel. The values are means ± S.E. (n = 3).
| [Zn2+] | T1 |
T2 |
T3 |
T4 |
||||
|---|---|---|---|---|---|---|---|---|
| Time | Area | Time | Area | Time | Area | Time | Area | |
| ms | % | ms | % | ms | % | ms | % | |
| Open lifetime parameters | ||||||||
| Control | 1.0 ± 0.05 | 89.0 ± 3.0 | 5.0 ± 0.9 | 11.0 ± 3 | ||||
| 100 pm | 1.0 ± 0.1 | 88.0 ± 1.2 | 4.6 ± 0.6 | 12.0 ± 1.0 | 15.0 ± 8.0 | 2.0 ± 1.0 | ||
| 1 nm | 1.0 ± 0.1 | 62.9 ± 9.0 | 4.5 ± 0.78 | 32.0 ± 8.0 | 27.0 ± 4.0 | 4.6 ± 2.5 | ||
| 100 nm | 1.2 ± 0.3 | 55.0 ± 14.0 | 5.2 ± 0.84 | 34.0 ± 6.0 | 21.2 ± 4.5 | 10.6 ± 9.0 | ||
| 100 μm | 2.2 ± 0.4 | 46.5 ± 0.9 | 10.2 ± 3.6 | 43.0 ± 8.0 | 60.6 ± 20 | 10.2 ± 6.8 | ||
| Closed lifetime parameters | ||||||||
| Control | 1.1 ± 0.1 | 85.8 ± 3.9 | 5.9 ± 0.9 | 39.3 ± 0.5 | 25.3 ± 1.8 | 24.6 ± 6.3 | 170.0 ± 12.7 | 6.8 ± 3.1 |
| 100 pm | 1.0 ± 0.1 | 72.0 ± 2.3 | 5.3 ± 0.6 | 26.0 ± 2.6 | 46.0 ± 15.0 | 2.3 ± 0.3 | ||
| 1 nm | 1.0 ± 0.0 | 75.0 ± 5.3 | 6.0 ± 1.7 | 21.6 ± 5.5 | 42.5 ± 13.4 | 3.0 ± 1.0 | 184.0 ± 27.0 | 1.3 ± 0.6 |
| 100 nm | 1.1 ± 0.1 | 48.0 ± 19.8 | 4.7 ± 1.2 | 36.5 ± 3.7 | 31.1 ± 1.3 | 15.0 ± 18.0 | ||
| 100 μm | 1.0 ± 0.1 | 62.3 ± 11.1 | 6.4 ± 2.4 | 30.3 ± 6.5 | 41.7 ± 17.2 | 7.3 ± 5.1 | ||