Figure 2. The TAD is necessary for muscle Ctnnb1 to regulate presynaptic development.
Diaphragms of P0 mice were subjected to electron microscopic analysis. (A) Representative electron micrographic images of indicated genotypes. Asterisks, active zones; SBL, synaptic basal lamina; N, nerve terminals; M, muscle fibers; SC, Schwann cells; SV, synaptic vesicle. (B–E) Quantitative analysis of data in A. Data were shown as mean ± SEM; **, p < 0.01; ***, p < 0.001, One-way ANOVA, n = 10.
Figure 2—figure supplement 1. Synaptic nuclear localization of Ctnnb1 and Axin2-nlacZ in the NMJ region.
Muscle fibers were stained whole mount with anti-Ctnnb1 (A, green) or for β-galactosidase (B, red), together with DAPI to label nuclei and R-BTX to label AChR. bar = 10 μm. NMJ, neuromuscular junction.