Figure 8. Slit2 was enriched at the NMJ and induced synaptophysin puncta of spinal cord explants.
(A) P12 tibilalis anterior muscles stained with R-BTX (red), anti-neurofilament antibody (green), and anti-Slit2 antibody (blue). (B) Detection of Flag-Slit2 in condition medium of transfected HEK293 cells. (C) Increased synaptophysin puncta co-localization with Flag-Slit2-conjugated beads. (D–E) Quantitative analysis of data in C, Data are shown as mean ± SEM; **, p < 0.01; t-test; bar = 10 μm for A; bar = 500 μm for C.