Figure 2.

Notch- and Alk1-mediated control of Notch target gene expression is gene-specific and context-dependent. Whole mount in situ hybridization for tp1:egfp, hey2, efnb2a, dll4, and cdh5 in 36 hpf wt and alk1^ft09e embryos treated with 1% DMSO or 10 μmol/L LY411575, 23–36 hpf. (A) Trunk. Lateral view, anterior leftwards. (B) Head. AA1, white arrow; ICA, blue arrowhead; LDA, white asterisk; CaDI, white arrowheads; OA, blue asterisks; MtA, black arrowheads. tp1:egfp, efnb2a, cdh5: frontal views, anterior up. hey2, dll4: lateral views, anterior left. Numbers in upper right corners indicate number of embryos with similar phenotype/total number of embryos assayed. Scale bars, 100 μm.