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. 2015 Jul 10;5:12095. doi: 10.1038/srep12095

Figure 4. Effects of GALR2/3 antagonists on spexin-induced intestinal and colonic motility.

Figure 4

The jejunum tissues were allowed to equilibrate for 1 hour and then treated with GALR2 antagonist M871 (0.05–50 nM, A &C) and GALR3 antagonist SNAP37889 (0.01–10 μM, B&D). The colon tissues were allowed to equilibrate for 1 hour and then treated with GALR2 antagonist M871 (50 nM, E) and GALR3 antagonist SNAP37889 (10 μM, F). 30 minutes later, the tissues were treated with spexin (1 μM) and the mechanical activities were recorded using the POWERLAB system and CHART5 software. The Emax% of 1 μM spexin in jejunum were calculated. Statistical differences between individual groups were evaluated using One way ANOVA. *P < 0.05 and **P < 0.01 compared with paired saline-treated controls.