Regulation of Growth and Gene (GA20ox and GA2ox) Expression by GA in the Transgenic Tomato Overexpressing the Arabidopsis RGA∆17 Mutant Gene.
(A)
qRT-PCR analysis of RGA∆17 expression in M82 and transgenic tomato plants. RNA was extracted from young leaves of the T2 generation. Values (gene-to-TUBULIN ratios) are means of three biological replicates ± se.
(B) M82 and transgenic RGA∆17 plants treated with 100 μM GA3 three times a week for 2 weeks.
(C) and (D)
qRT-PCR analyses of GA20ox1
(C) and GA2ox4
(D) expression in tomato leaves treated with 10 mg/L PAC for 3 d or PAC for 3 d followed by one application of 100 μM GA3. Values (gene-to-TUBULIN ratios) are means of three biological replicates ± se.