Figure 3.

Homodimerization of WKS1.1 and WKS1.2 in Y2H Assays Using Complete and Partial Protein Segments.

(A) Protein regions used in Y2H assays: full-length (FL), kinase domain alone (KA), kinase with interdomain (KI), interdomain with START domain (IS), and START domain alone (SA). Primers are indicated by horizontal arrows and listed in Supplemental Table 2.

(B) WKS1.1 autoactivation test. BD, binding domain; AD, activation domain.

(C) Interaction between complete WKS1.1 and complete WKS1.2 or truncated WKS1 proteins.

(D) Homodimerization of WKS1 domains.

(B) to (D) Positive control = pGBKT7-53/pGADT7-T; negative control = pLAW10/pLAW11. See Supplemental Figure 3 for mutants and Supplemental Figure 5 for control protein gel blots.